Direct skeletal attachment of lower limb prostheses ensures direct load transfer between the prosthetic leg and the skeleton. Knowledge of the load characteristics at the boneimplant interface during high-loading activities is needed to understand the limitations of current implant systems, as well as to inform their future development. The present study estimates the load scenario at the bone-implant interface of a transfemoral amputee while running with kinematic symmetry between the prosthetic and the intact limbs corresponding to that of an ablebodied subject. Kinematic symmetry was used as this represents the ultimate aim of advanced bionic legs. Kinematic data and ground reaction forces from a running trial of an able-bodied subject were matched to a musculoskeletal model of a transfemoral amputee. The joint reaction forces at the boneimplant interface were calculated using inverse dynamics. The normalized peak forces and moments during a single gait cycle were calculated to 153 % BW (body weight) / -14.8 % BWm, 186 % BW / 16.2 % BWm and 56.8 % BW / -18.7 % BWm for the x- (anterior), y- (longitudinal), and z-axis (lateral-medial), respectively. These findings can potentially be used as design input for future implant systems and external safety devices.